Doubled Haploids via Microspore Embryogenesis in Tomato

Tomato (Lycopersicon esculentum L.) microspore embryogenesis and doubled haploid production technology is not available so far and the only promising results were obtained in the founder?s laboratory. MicroCrop continue the research program to establish tomato ME and DH production and technology is expected to be ready for large scale use by the end of the second or even third year of company business activity. Established technology will be subject for IP and the technology will be licensed on non-exclusive basis to interested companies and also used for custom production of tomato homozygous lines.

Progress, made in MicroCrop: MicroCrop has achieved major breakthrough during the last years in inducing large number of in vitro cultured tomato microspores towards sporophytic division and formation of pro-embryo structures (Fig. 1 and 2). Almost 70% microspores could be induced to divide and form multicellular structures. Important to note, that among more than 20 different lines, varieties, species tested, all demonstrated similar response.

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Figure 1: In vitro development of isolated tomato microspores into multicellular structures at high frequency

 

 

 

 

  

 

 

 

 

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Figure 2: In vitro development of isolated tomato microspores into multicellular structures and pro embryos